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Description
SMAD3 Recombinant Rabbit mAb (S-3147-36)Product Specification Host Rabbit Antigen SMAD3 Synonyms Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD 3; JV15 2; SMAD family member 3 (SMAD 3; Smad3; hSMAD3); MADH3 Immunogen Recombinant Protein Location Cytoplasm, Nucleus Accession P84022 Clone Number S 3147 36 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC Reactivity Hu, Ms, Rt, Mk Positive Sample HT 1080, HeLa, Jurkat,
Product Specification
| Host | Rabbit |
| Antigen | SMAD3 |
| Synonyms | Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3 (SMAD 3; Smad3; hSMAD3); MADH3 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | P84022 |
| Clone Number | S-3147-36 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | HT-1080, HeLa, Jurkat, K562, A431, RAW264.7, C6, COS-7 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| IHC-P | 1:1000 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
SMAD3 protein is a key intracellular signaling transducer in the Transforming Growth Factor-β (TGF-β) signaling pathway. As one of the receptor-regulated SMADs (R-SMADs), SMAD3 is phosphorylated and activated upon binding of TGF-β superfamily ligands (such as TGF-β and activin) to their cell membrane receptors. It then forms a complex with SMAD4 and translocates into the nucleus, where it functions as a transcription factor to directly regulate the expression of target genes. It is extensively involved in physiological processes such as cell proliferation, differentiation, apoptosis, extracellular matrix formation, and immune regulation. SMAD3 plays a dual role in various pathological conditions, including fibrosis, cancer, and inflammatory diseases (it can suppress early tumor growth but also promote late-stage tumor progression and metastasis). Its activity is finely regulated by post-translational modifications such as ubiquitination and acetylation, as well as by inhibitory SMADs (e.g., SMAD7), making it an important potential therapeutic target for diseases.
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