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Description
Rat MuRF1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Muscle Ring-Finger Protein-1 (MuRF1). After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Muscle Ring-Finger Protein-1 (MuRF1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Muscle Ring-Finger Protein-1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The E3 ubiquitin-protein ligase TRIM63, also known as "MuRF1," is encoded by the TRIM63 gene. This gene encodes a member of the RING zinc finger protein family, found in striated muscle and iris. The gene product is localized to the Z-line and M-line lattices of myofibers, where the N-terminal and C-terminal regions of titin, respectively, bind to the myofiber. In vitro binding studies have shown that this protein also directly binds to titin near the region containing kinase activity. Another member of this protein family binds to microtubules. Because these family members can form heterodimers, this suggests that these proteins may serve as a link between myosin kinase and microtubule-dependent signaling pathways in muscle. MuRF1 is the most commonly used name in the literature, standing for "muscle RING Finger 1." Structurally, there are two closely related MuRFs, MuRF2 and MuRF3. These also have the TRIM code. MuRF2 is TRIM55, and MuRF3 is TRIM54. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.9 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
Well-packaged, quality materials, easy to assemble, very comfortable, great customer service
Color: Black Full Mesh, Style: Executive
When I received this package, I was surprised at how small/compact it was--hard to believe a high-back office chair could fit in that box! As I unpacked it, I was impressed by two things: first, how incredibly well it was packaged with an obvious intent to protect the components; second, how solid, heavy-duty, and high-quality those components were.
I got the box unpacked and read the instructions. They were simple and easy to understand, and included so many "here's a good tip" notes that it was obvious that a lot of thought and experience had gone into writing them. I found the chair simple to assemble and had it done in less than 15 minutes.
The chair was very adjustable. I was able to get the height to exactly what I wanted with no trouble at all. I was also able to adjust the height of the lumbar support, and to adjust how far it extended. There are a lot of adjustments you can make, the instructions lay that out quite thoroughly, too. As a result, the chair was very comfortable and helped me sit with a healthy posture.
I bought this chair because I had begun having an ache in my lower back, which I suspected was due to a chair I had been using for over 5 years. Within 3 days, the back pain was gone.
I was also very impressed with the customer service. Their manual mentions in multiple places things you can try if you have any issues, and always ends with "call us". Their team reached out to me after a few days to make sure I got the chair assembled with no issues and that it was comfortable. This was all part of the all-around quality of this purchase.
I'm very happy with this chair!
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Reviewed in the United States on June 5, 2026
★★★★★ 5
Comparable to Higher-End Chairs, Solid Build and Support Without the Premium Price
Color: Black Full Mesh, Style: Executive, Color: Black Full Mesh, Style: Executive
I’ve been using this chair for a bit now and it’s been a genuinely upgrade. The build quality stands out right away, it has real heft and doesn’t feel cheap or hollow. Everything fits together cleanly and once assembled it feels stable and planted. I’m over 6 ft and 200+ lbs and it handles that without any issue, no creaking or sense that it’s being pushed to its limits.
There’s enough space in the seat and back that you don’t feel boxed in. I added an extra ergonomic cushion and it still feels roomy, which isn’t always the case with chairs in this category. The adjustability helps a lot here, especially the lumbar support. Being able to move it up and down and also forward and back makes it easy to actually get it where you need it, instead of just accepting a fixed position. The armrests are also easy to dial in, especially if you’re switching between typing, reading, or leaning back a bit. The tilt range is useful, and the tilt tension is stronger than I expected. Even at my size it takes a bit of effort to lean back, which I see as a positive since it feels controlled and supportive rather than loose or overly easy to recline. The mesh is another strong point. It came in perfect condition, no snags, no loose threads, no rough edges anywhere. There weren’t any bits of untrimmed plastic on the frame either, which says a lot about the quality control. The mesh itself is taut with a bit of bounce, so it doesn’t sag but still has some give, which makes long sitting sessions more comfortable.
Assembly was straightforward and clearly thought through. The instructions are easy to follow, but what stood out were the built in redundancies that keep you from second guessing anything. There are multiple left and right indicators when installing the arms, a clear front facing marker for the gas lift, and the screws are labeled in the packaging so you’re not guessing which goes where. It’s one of the smoother assembly experiences I’ve had.
I also reached out to activate my warranty and had a very positive experience. I spoke with Alice who was very knowledgeable, quick, warm, and they went out of their way to help me with whatever I needed. When I inquired about the extra screw holes under the chair, she let me know that this was for a foot rest which was still in the production phase and unprompted offered to have one shipped to me when they were ready! There is an excellent team behind the product.
Overall: well built chair with good materials, thoughtful design choices, and good adjustability to make it work for different setups. It feels durable, comfortable over longer periods, and especially well suited if you need something that can handle a larger frame without sacrificing support.
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Reviewed in the United States on April 20, 2026
★★★★★ 5
Great product AND customer service!
Color: Black Full Mesh, Style: Executive
I'm having a hard time writing this review because I can't tell which one I like better: the product or the customer service!
I work on a computer all day, so a comfortable office chair is critical for me. This chair is great - it's adjustable in every way I need it to be, especially the armrests. The breathable mesh is both durable and supportive, and is more comfortable to sit in than the "overstuffed" chair it replaced.
Let me just say: no company provides better customer service than MOLENTS. Right after assembling my chair (which was very easy, btw), I registered for the 2 year warranty they offer. The NEXT DAY I received an email from Alice in their customer service department 1) to confirm my warranty, 2) to tell me about the free foam seat cover they were sending me, and 3) to make sure the chair was comfortable and easy to assemble. Very few companies take the effort to follow up with customers right after making a purchase like that.
I'm extremely happy with this purchase. This chair is worth MORE than it's weight in gold, and I strongly recommend to anyone thinking about buying an office chair to buy it from MOLENTS - you won't regret it.
Oh, and if Alice's supervisors are reading this: she absolutely deserves a promotion!
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Reviewed in the United States on March 13, 2026
★★★★★ 4
good chair for price
Color: Black Full Mesh, Style: Executive
I have had for about 2 months and seems to be holding up well, so far, and is comfortable for most part. Only complaint is the arms do not lower a lot, so cannot go under the desk.
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Reviewed in the United States on April 29, 2026
★★★★★ 5
High quality and incredible customer service
Color: Black Full Mesh, Style: Executive
This is an extremely well made chair at a very competitive price. The packaging, assembly instructions, as well as the way the hardware was packaged and labeled is probably the best I've seen. I'm particular about tools, and the included allen wrench was better than the normal "included tools" so much so that i actually used it rather than one of my own. The assembly was easy, and the chair itself looks and feels very high quality, and is very comfortable. Perhaps best of all was the level of customer service. I emailed on a weekend to activate the warranty and request the free cushion they offer. I got a personal response in less than a day and they offered upgraded wheels for free as well. This is a company worth doing business with.
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Reviewed in the United States on April 5, 2026
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