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Description
EdU-647 cell proliferation detection kitProduct Specification Usage Before the trial guidelines 1, the corresponding animal experiment, EdU suggested initial dosage of 50 mg kg (50 mu g g), dilute concentration is 0. 5 1 mg mL. Are injected into mice weight 20 g, mg, 1 to 1 mg mL, need injection of 1 mL. 2, for the cells, the recommended 10 microns, EdU work for in vitro culture cell, specific EdU use concentration, incubation time along with the samples and research purpose is different,
Product Specification
| Usage | Before the trial guidelines 1, the corresponding animal experiment, EdU suggested initial dosage of 50 mg/kg (50 mu g/g), dilute concentration is 0.5 1 mg/mL. Are injected into mice weight 20 g, mg, 1 to 1 mg/mL, need injection of 1 mL. 2, for the cells, the recommended 10 microns, EdU work for in vitro culture cell, specific EdU use concentration, incubation time along with the samples and research purpose is different, can be adjusted appropriately. 3, this kit can be carried out about 200 t of 6 orifice EdU experiments, about 2000 t96 orifice or paraffin section EdU experiment, experiment should be calculated according to the sample situation in the process of usage. I. Sample processing 1, the animal experiment (1) EdU injection in animals In mice, can according to the amount of 10-200 mg/kg, the EdU with PBS mixed with a certain concentration, intraperitoneal injection, specific tissues or organs local injections or added to drinking water. The specific dosage is related to the type, weight and mode of use of animals, and can be referred to the relevant literature. Therefore, it is recommended to conduct a certain exploration of the use concentration of EdU for the first use, or directly use the concentration of 50mg/kg for testing. Injection method: the experiment according to the customer, such as abdominal cavity injection, subcutaneous injection, intramuscular, tail intravenous injection, etc., among them with intraperitoneal injection. After 6 h or after the appropriate time was determined according to the specific experiment, the mice were quickly sacrificed, the desired tissue was removed, and frozen or paraffin sections were made according to routine procedures. EdU labeling can also be adjusted by referring to the relevant literature. Small intestinal epithelial cells proliferate rapidly, and positive information can be detected 6 hours after EdU injection in adult mice, which can be used as a positive control for pre-experiments. (2) Slice processing Pre-section treatment: It is best for tissues and organs to be washed to remove residual EdU in blood and tissues and reduce background. Section thickness: 3-10μm is appropriate, too thick section may affect the section background and staining efficiency. Slice post-processing: Paraffin sections were deparaffinized twice with xylene for 15 min, once each with gradient ethanol (100%, 95%, 85%, 75%) for 5 min, and once with deionized water for elution. Frozen section processing: room temperature placed after 30 minutes, fixed 10 minutes, PBS cleaning three times, each time for 5 minutes. 2. Cell experiments (1) cells EdU markers a.with cell complete medium and EdU mother liquor by 2000:1 the ratio of dilution, preparation of a moderate amount of 10 microns EdU medium; b.each hole adding suitable amount of 10 microns EdU medium 2 hours incubation, abandon culture medium (optimal incubation time for 1/10 of the cell cycle) commonly, EdU incubation medium and EdU reaction liquid volume may refer to the table below; c.PBS cleaning cell 3 times, each time for 5 minutes.
a. Cell fixative (PBS containing 4% paraformaldehyde) was added to each well and incubated at room temperature for 15 minutes. The fixative was discarded and washed with PBS for 3 times, 5 minutes each time. b. Each well was decolorized with an osmotic agent (0.5% TritonX-100 in PBS) and incubated for 15 minutes. PBS was washed three times for 5 min each. Second, EdU response
The EdU reaction solution was prepared according to the ratio of PB: CU: AC: 647 chromogen = 855:40:100:5 (the reaction solution was prepared immediately). Each sample add 50-500 mu L EdU staining reaction liquid liquid evenly cover samples (reaction), 15 to 60 minutes at room temperature away from light incubation, abandon the dyeing liquid reaction, PBS wash 3 times, each time for 5 minutes. 3. DAPI staining DAPI(100x) was diluted with PBS at a ratio of 1:100 to make ready-to-use DAPI staining solution. 50-500μL of DAPI staining solution was added to each sample and stained in the dark for 15 minutes, followed by three washes with PBS for 5 minutes each. 4. Take pictures Fluorescence microscope, confocal microscope or whole-film scanner were used to collect images. After staining, the slides were stored in a dark place at 4℃. Above are stained images of 50mg/kg labeled mice for 12 hours |
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| Description | EdU(5-ethynyl-2' -deoxyuridine), also known as 5-ethynyl-2' -deoxyuridine (5-ethynyl-2' -deoxyuridine), is a thymidine analogue with an alkynyl hydroxyl group that is rare in natural compounds and can replace thymidine (thymidine, 5-ethynyl-2' -deoxyuridine) in cell proliferation. thymidine (thymidine) is inserted into the replicating DNA molecule, the ethynyl group on EdU can react covalently with the fluorescently labeled small molecule Azide probe (Azide Alexa Fluor 488, etc.) through the catalysis of copper monovalent ion to form a stable triazole ring. "It is called the Clickreaction, which allows for an efficient and rapid measurement of cell proliferation, especially the percentage of cells in S phase." With traditional immunofluorescence staining (BrdU) method compared with EdU only 1/500 the size of the BrdU antibody, are more likely to spread within the cell, don't need strict sample degeneration (acid solution, pyrolysis, enzyme solution) processing, effectively avoid the damage samples, help in the overall level of tissue and organ observation of cell proliferation, It has higher sensitivity and faster detection speed.Note: AC is a powder, need to add 10mL pure water before use, fully dissolved before use, AC is easy to oxidation failure, after the refrigerator is stored at -20 degrees, if found discoloration, need to replace fresh reagent. In animal experiments, the mass concentration of 100mM EdU mother liquor corresponds to 25mg/mL, which should be converted according to the dosage.
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| Storage Temp. | -20 ° C, valid for 12 months. |
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4.3 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 5
Outstanding Indie Fantasy! You NEED to read this!
I have started and deleted my intro to my review multiple times at this point. My mind is sufficiently BLOWN. This book has landed itself into my top favorite romantasies right alongside ACOTAR and FBAA. ITS SO DANG GOOD. I literally woke up, popped it open on my kindle app (Thanks author and R&R Book Tours for the arc!) and got to reading and just didn’t stop. I couldn’t stop. From the first dang page, Menard dragged my butt in and tied me down. I. Was. HOOKED.
Writing Style: I absolutely love the writer’s style! Told from mainly Milla’s POV, with the occasional glance into Nico’s POV when its important. Her world building is rich and immersive, sticking firmly into the steampunk/gas lamp fantasy atmosphere. Her magic system—while feeling familiar—still feels unique and blends beautifully with the world she’s created.
Characters: I can’t begin to describe how much I love the characters Menard has created for this story. Milla is a firestorm. She’s passionate, fiesty, loyal, and self confident. She doesn’t back down, no matter the odds. For me, personally, she’s ranking right up there with Feyre (ACOTAR), Poppy (FBAA), and Sera (FBAA prequel) as one of my favorite female leads. Despite her fierceness, she’s not invincible and she’s still vulnerable—which unveils itself more as the story goes on.
Nico, mmmmmmmm, Nico. He’s cocky, and strong. His heart is for his blood and family. There’s nothing he won’t do for those he cares for. He’s incredibly insightful which leads to a lot of me squeeing and highlighting his quotes. Pairing him up with Milla is a riot I will watch again and again.
The side characters are all phenomenal, ranging wide in personalities and giving the world a richer vibrancy. They compliment both main characters, bringing out the best and worst in them, helping to bring secrets to light and keep the plot going. Not a single character felt out of place, or ever out of character.
Relationship: Y’all know I love a good slow burn and this burns so good. The push and pull of their hate/love relationship is a thrill to witness. Watching them fall for each other is a joy and the spice (when we get there) is HAWT.
Plot: This story had me hooked, and that’s largely in thanks to how well planned out the story’s plot is. Menard did an excellent job, masterfully balancing her foreshadowing and reveals. While I halfway guessed the big reveal, i definitely did not expect the big reveal. (Read the book, then you’ll understand lmao). The pacing is fantastic. Nothing about the story felt rushed, not the relationship, not the reveals, not the over arching plot line. Just absolutely phenomenal.
Enjoyment: 100/10 will read again. I need a physical copy in my life. I HIGHLY recommend this book.
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Reviewed in the United States on October 28, 2023
★★★★★ 5
One of the best reading experiences I’ve ever had
Format: Audiobook
This was truly AMAZING!!!!!!!!!!!! In every possible way.
- the setting. I loved the steampunk/gaslamp setting with trains, overcast weather, and mafia vibes
- the magic. I still don’t entirely understand the magic system and descendants as much and it took awhile for me to get my footing in that department- and I am intrigued beyond measure, though it in no way lessened my enjoyment of the story. I just really wish I had a better grasp of it.
- the plot itself. Beautiful. The tropes were troping in the absolute best way. Every single thing that happened I was like YES MORE!!!
- the writing. I found myself grinning ear to ear uncontrollably during my entire read through and full on choking on my gasps, again, like the entire read through. And I read this too at times when I was in an awful mood so the way this book was an INSTANT pick me up really speaks to how well this was written. I was having the absolute best time, non stop.
- the characters. The best part oh my gosh. Camilla is SUCH a badass. Her and Nico are supposed to be low 20s and I gotta tell you they read more like 30s from how capable and mature they are (not that that age bracket is incapable or immature, just comparing them to other characters written in that age range). She is the type of cool as heck character that everyone would be cos-playing as and if it was a kid movie, every single girl would dress as her for Halloween. She’s that awesome.
Nico is top freaking tier. I gotta say his hairstyle is my undoing and with the addition of the metal arm- before I even started reading the book I was in love with him. And he was EVEN BETTER than my lofty expectations. This man. Perfect in every single way. Every. Single. Way. If I start now I won’t stop so I’m just gonna leave it at that. Camilla and Nico are power couple of the century though and the way they interacted was the most toe-curling/squealing/kicking your feet banter I’ve read in a while. PLUS the way they fell for each other was a gem. I was falling in love with their relationship right along with them.
- a shout out to the side characters too. Aramis, Gideon, Aunt Fran, Esme, Luther, Adler [honestly just insert all the supporting cast they were freaking phenomenal] brought so much life and personality to the story and helped round out Nico and Camilla. I really really really would absolutely adore if Adler got some more spotlight time in the future because he and Aramis are up there with “let’s give these side characters their own spinoffs”.
Also, the villains were great too. I love a great villain in a story and sometimes I don’t think they get highlighted as much in books as in movies or TV that I hear of.
If it wasn’t obvious already- this was even greater than a 5 star read. This was perfection- in quality and in vibes- and it was one of the most enjoyable reading experiences I have ever had and I’m not throwing that around lightly. I have scenes from this book playing in my mind unbidden on repeat and I know this world has so much more to be explored. I will read anything Alexis L Menard puts out in the future
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Reviewed in the United States on March 5, 2025
★★★★★ 4
Great mix of maffia and magic
Format: Kindle
This one felt really unique to me, with a great and successful blend of Twenties-feeling maffia and inventive magic. I liked both the main characters a lot, even though I found myself almost shouting at the MFC sometimes. She really should learn not to trust everyone just because they're family!
All in all a good read, with lots of romance, yearning and some spice as well.
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Reviewed in the United States on November 15, 2025
★★★★★ 3
So close to being good
I really wanted to like this book. It had all the makings of a strong, interesting story, but the plot got so convoluted that it was hard to follow. Also, for a "badass" FMC, Milla was amazingly inept. The writing got really stilted at times too. I will probably still read the next one anyway, because I'm curious to see where the plot goes.
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Reviewed in the United States on May 3, 2024
★★★★★ 5
FASCINATING world, great characters and swoony slow burn!
House of Bane and Blood by Alexis Menard
-Order and Chaos Book 1-
4.5/5⭐️
1/3🌶️*
2/3🦋
•My Thoughts•
What a RIDE! This world was absolutely fascinating with its 1920s mafia vibes meets magic. The characters and enemies to lovers storyline hooked me from the beginning - add in a marriage of convenience and a dash of mystery and I ate👏🏻it👏🏻up👏🏻. Milla’s growth was so good and I was cheering for her as she became more confident! I also loved Niko and his back story and resilience. These are imperfect characters you find easy to love, root for and I became enamored with their slow but steadfast (if reluctant) support of each other. I did find the government structure a little confusing but didn’t want to slow down enough stop and figure it out (if anyone reads it an wants to make a chart, please share it with me). This book was violent but also mesmerizing and full of hope for a better future. I loved the big family dynamics and the never ending plot twists that kept me guessing. This is a completed duology and with that ending, you BET I’m immediately reading book 2.
*I rated this a 1/3🌶️ because there were only a few scenes with on page intimacy. However, I would like to note that the spice involves kink: breath play.
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Reviewed in the United States on June 10, 2024
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