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Description
Bovine Mammary Glands Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.0 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 5
Soulless Saint
Format: Kindle
If you're looking for a dark, thrilling romance that will keep you on the edge of your seat, "Soulless Saint" by Elena Lawson is the book for you. This first installment in the Kings of Kilborn University dseries is an irresistible mix of passion, danger, and intense chemistry.
The story follows Becca, who moves to a new city to escape her past, only to find herself entangled with Kaleb and Hardin, crime lords known as the Sainted Kings. Initially tasked with protecting her, their relationship quickly evolves from animosity to an obsessive attraction that neither can resist.
Becca is our fiery fmc and she is trying to rebuild her life after being cut off by her father. Kaleb and Hardin are complex and compelling—Kaleb is haunted by his own demons, while Hardin is the silent, deadly type. Their interactions with Becca are electrifying, filled with tension and undeniable chemistry.
The book is packed with steamy scenes, raw violence, and unexpected twists that will leave you breathless. Just when you think you know where the story is going, Lawson throws in surprises that keep you hooked. The introduction of Aódhan, a mysterious and captivating character - needing more of him.
"Soulless Saint" is a fast-paced, gripping read that you won't be able to put down. If you love dark romance with multifaceted characters and high stakes, this book is a must-read. The ending will leave you eagerly anticipating the next book.
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Reviewed in the United States on June 19, 2024
★★★★★ 5
"In a moment of clarity, I realized I knew why a good guy would never be enough..."
Format: Kindle
"...Good guys didn't touch you like the bad guys did. They didn't push the limits. Hold you down. Make you insane with need. Make you scream."
A spin off from the Crows but it's own story. I've been rushing to get to this book (had some others I had to read first). But I could not hold off any longer. I happily picked it up and refused to put it down. This book is going to pick up where we left off with Becca from the Boys of Briar Hall. She has made her way to SoCal Arts and cut off by her dad.
The Kings of Kilburn, also cousins to our Crows, have been asked to watch Becca as a favor. Ava Jade is worried about her. Hardin and Kaleb have their own issues but someone find themselves individually drawn to Becca. And Becca, well her bad guy rep is pulling her to them just as much. What should be toxic, is super HOT.
But the Kings have Saint business as well. Damian St Vincent, the ruthless leader of this Saints chapter, also happens to be the boys father. And they are currently being threatened by a new Irish gang, Sons of Sullivan. This threat is unaliving member of the Saints and messing up relationships that have been established. Damian warns the guys to stay away from Becca because with this threat, it could put her in danger if she is a known associate to the boys. But things are never that easy.
And lastly, we have would could be a third player. Aódhan. He's Irish and has his own secrets. Also super hot and seems to find himself infatuated with Becca too. But the last chapter, we are going to see inside his POV, and there is more to this Irishmen to come for sure.
Cliffhanger! I always want more from Elena Lawson books, but I love them so much I can't wait for the series to be completed before diving in.
**READ YOUR TRIGGER WARNINGS**
Happy Reading Ya'll!
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Reviewed in the United States on April 6, 2023
★★★★★ 3
hoping for a little more
Format: Kindle
I was hoping for a little more with this book as I loved the other series in this “world,” and hoping it’ll come in the 2nd book. During this book I kept skipping pages ahead trying to move things along, just didn’t really feel like much was answered or accomplished but I’m sure that was the authors point in trying to build everything up but for me it just dragged a bit…Will absolutely read book 2 to see how it all unfolds.
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Reviewed in the United States on July 2, 2024
★★★★★ 5
Can’t Wait For The Next Book!!!
Format: Kindle
⭐️⭐️⭐️⭐️⭐️
Review
This is the first book in the new series Kings Of Kilborn University by Elena Lawson and an absolutely fantastic start to the series. This follows after Boys Of Briar Hall Series and we even get to see AJ and the Crows.
This is Becca’s story after she left Thorn Valley and it’s one intense ride to say the least. Trying to avoid the gang violence she previously suffered she gets a fresh start and a chance at independence something she’s been desperate for. When she meets brothers Kaleb and Hardin things get absolutely intense. They are the Kings of Kilborn and also her new tormentors and they become absolutely obsessed with her. This is a story you have absolutely got to read. The chemistry between the three is off the charts even though Becca just wants to keep away from them she can’t deny the connection.
This book will seriously have you on edge, it’s got violence, gang war between the Saints and the O’Sullivan Sons, lots of twists and turns, and unexpected revelations at the end that will leave you needing book two asap. Am seriously looking forward to finding out what happens next for these characters. Definitely an awesome start to new series. Highly recommend this.
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Reviewed in the United States on February 6, 2023
★★★★★ 4
4.5 ⭐ I loved it!
Format: Kindle
Excellent story line.
I have loved all in this book whisch was very smart.
My favorite gentleman here is Aodhan since the begining I fell for him.
The sex chemistry here was amazing!
The only thing I did not like was: they talk about class in the university but we never had a scene in that scenario the rest very good.
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Reviewed in the United States on October 1, 2025