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Description
Mouse IL-16 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 pg/mL). Then dilute to the following concentrations: 200 pg/mL, 100 pg/mL, 50 pg/mL, 25 pg/mL, 12.5 pg/mL, 6.25 pg/mL, 3.125 pg/mL, and 0 pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 200pg/mL standard working solution into the first EP tube and mix thoroughly to make a 100pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 16 (IL-16) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 16 (IL-16) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 16 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-16 (IL-16) is a protein encoded by the IL16 gene. The protein encoded by this gene is a multifunctional cytokine with functions as a chemoattractant, a regulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The gene product undergoes proteolytic processing, resulting in two functional proteins. The cytokine's function is entirely attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control. Caspase 3 has been reported to be involved in the proteolytic processing of this protein. Two alternatively spliced transcript variants have been reported to encode different isoforms. Interleukin-16 (IL-16) is a cytokine released by a variety of cells, including lymphocytes and some epithelial cells. It is characterized by its chemoattractive effect on certain immune cells that express the cell surface molecule CD4. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.0 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 5
Great Value, Compact, & Reliable Mous
Color: Blue, Style: 1-Pack
The Amazon Basics 2.4Ghz mouse works well right out of the box. I really appreciate its compact size, as it doesn’t take up much space on a mouse pad or flat surface—great if you’re working in a limited area. Its ergonomic design also feels comfortable under the hand. Overall, I think it offers good value for the money, and it’s very easy to set up and use. So far, the battery life has been solid—I’ve used it for three months and it’s still going strong. I put in Amazon Basics battery in this mouse, and surprisingly it lasting a long time.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 17, 2026
★★★★★ 5
A Simple, Effective, and Inexpensive Device
Color: Silver, Style: 1-Pack
I have tried many variations of the standard computer mouse over the years. You probably have too. From weird angles, extra buttons in the scroll, funny side buttons that only make you go backwards on your web browser...Sometimes, you just want a point, click, scroll...and that's it.
Pros:
- This mouse delivers on simplicity. It doesn't have fancy advanced features. This basically functions as a point and click. It has a left and right hand button to do those old fashioned right and left button tasks. It has a basic scroll button. It's low tech features, but quality built for those features that it has.
- It seems to work on most surfaces without a mouse pad.
- Cost is ridiculously low. It's almost so low that you question its quality. In this case - it's Amazon. Amazon has really delivered on their Amazon Basics division. This falls neatly into that category of awesome but inexpensive Amazon Basics stuff.
- Connected within a second to my computer (Mac). I don't see any connectivity issues. I didn't have to download drivers at all.
Cons
- I haven't used it long term yet so if there is a break down, or the battery life turns out to be poor, I will update. For now, it's behaving beautifully.
Overall, if you miss a nice, simple, uncomplicated mouse - start here. Worst case scenario, you can return it. After all, Amazon has been standing behind their stuff for a while now so I'm not afraid to start with theirs and see how well they did. This is another one that didn't disappoint and it cost 50% less than its nearest competitor (at the time I bought it at least).
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Reviewed in the United States on February 18, 2019
★★★★★ 5
This works well for me for work and gaming
Color: Blue, Style: 1-Pack
A very good travel mouse. It's not too big and the battery lasts a long time. It is comfortable and works well
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Reviewed in the United States on April 18, 2026
★★★★★ 4
Mostly a Really Good Mouse
Color: Silver, Style: 1-Pack
This mouse connected perfectly, feels good, and even works seamlessly with my cordless keyboard, which of course uses a different dongle. I have only one small issue, but it's one I thought worth mentioning. While the buttons work normally when moving the cursor around the screen, when it comes to actually making a selection with the primary button (right- or left-handed, doesn't matter), one must press the button VERY deliberately and somewhat slower for the "click" to be recognized. A typical "quick click" at the speed one would normally use, say, when typing or normal "mousing" often will not register with the computer. The result is that I have to slow down and click again. In several decades of using a computer mouse I've never experienced this phenomenon. I've grown used to it, however, and adjusted to the necessity to give the selection button a slower and harder "press" when making selections, and that's all it takes. And oddly, it only requires this extra deliberate action when pressing a selection button. Not the biggest issue one could have, but I thought it worth mentioning. Other than that, no problems, and really, the price is very good, so as they say, you pays your money and you makes your choice. ;-)
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Reviewed in the United States on March 23, 2025
★★★★★ 5
Great Value
Color: Silver, Style: 1-Pack
Works perfectly!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 23, 2026
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