SKU: 71107615457

Albumin /IgG removal kit

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Description

Albumin /IgG removal kitProduct Specification Usage Equipment preparation: centrifuge; Oscillator; Vortex mixer; Pipetting; The refrigerator; Ice boxes Reagent preparation: acetonitrile; Protein quantification kit Material preparation: centrifugal tube; Suction head; disposable gloves Method of use: 1. Other protease inhibitors can be added according to your own experimental needs. 2. The centrifuge speed has two ways of relative centrifugal force (RCF, g) and speed per

Product Specification

Usage

Equipment preparation: centrifuge; Oscillator; Vortex mixer; Pipetting; The refrigerator; Ice boxes
Reagent preparation: acetonitrile; Protein quantification kit
Material preparation: centrifugal tube; Suction head; disposable gloves
Method of use:
1. Other protease inhibitors can be added according to your own experimental needs.
2. The centrifuge speed has two ways of relative centrifugal force (RCF, ×g) and speed per minute (RPM). Some centrifuges have RPM and ×g display switching, but some centrifuges do not have automatic switching function. The following formula needs to be used for conversion: g=r×1.118×10-5×rpm2 (r is the effective centrifugal radius, that is, the length from the centrifuge axis to the center of the bottom of the centrifuge collection tube in centimeters)
For example, if the rotational speed is 3000rpm and the effective centrifugal radius is 10 cm, the relative centrifugal force (RCF, ×g) is =10×1.118×10-5×30002=1006.2 (×g).

Operation:
1, 200ul of the serum sample to be processed was centrifuged at 15000×g for 15 min at 4 ° C.
2, carefully remove the upper lipid layer, draw the middle tier serum 50 ul.
3, add in 150 ul reagent A, thoroughly incorporated.
4. Let stand at -20℃ for 30-90 min.
5, Centrifugation at 15000×g for 20 min at 4 ° C.
6. Carefully suck out the supernatant. The supernatant was discarded and the precipitate was retained.
7. 500ul of reagent B in an ice bath was added to the precipitate and mixed with gentle shaking. 15 min is placed on the ice.
8, Centrifugation at 15000×g for 20 min at 4 ° C. Carefully remove the supernatant, discard the supernatant, and retain the precipitate.
9. 500ul of reagent B in an ice bath was added to the precipitate and mixed with gentle shaking. Leave on ice for 15min.
10, Centrifugation at 15000×g for 20 min at 4 ° C. Carefully remove the supernatant, abandon supernatant, reservation precipitation.
11, precipitation will retain the centrifugal tube open let stand for 15 minutes. To dry.
12, add 100-400ul reagent C to the precipitate and mix thoroughly. Oscillation for 5-10 minutes.
13, centrifuged at 12000×g for 10 min at 4 ° C. The precipitate was discarded and the supernatant was removed.
14, in addition to the supernatant is the albumin serum protein samples.

Description

Albumin /IgG removal kit provides a full set of reagents suitable for rapid removal of albumin from serum/plasma samples.
Serum and plasma proteins are the focus of human index detection, and also the focus of scientific research. Commonly used techniques such as electrophoresis, 2D electrophoresis, liquid chromatography and mass spectrometry have been widely used in these studies. These techniques have limitations on the total amount of protein in the sample, and more than 50% of serum proteins are albumin, which will cause great interference to the detection of other low abundance proteins, which forces us to find ways to enrich the target protein and remove albumin.
The protein extracted by this kit can be used for downstream protein research experiments such as Western Blotting, protein electrophoresis, immunoprecipitation and ELISA.
The protein samples extracted by this kit contain a high concentration of salt components and cannot be directly used for 2D electrophoresis.
The protein extracted by this kit has an effect on the activity of the protein, and can not be used for downstream protein research experiments such as enzyme activity determination. 1. Simple, no special equipment other than centrifuge is required, and multiple samples can be processed in parallel.
2, high efficiency, one can effectively remove about 60% of the albumin, conducive to the detection of low abundance protein.

Composition
Component 50T Storage
Reagent A 7.5mL 2-8℃
Reagent B 50mL 2-8℃
Reagent C 20mL 2-8℃
Storage Temp. Store at 2-8 ° C for 12 months.
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Exchange/Return Notes
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SKU: 71107615457

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Aurora
Carnegie, US
★★★★★ 5
Recommended! A Must-Have for Sun Protection
Size: 1.5 Ounce (Pack of 1)
I've been using the Neutrogena Ultra Sheer Dry Touch SPF 50 Mineral Sunscreen Stick for a while now, and I can't imagine going out without it. This convenient stick sunscreen provides excellent sun protection without leaving behind a greasy or sticky residue. One of the things I love most about this product is the way it sits on my skin. It gives a subtle, healthy-looking glow that's far from chalky or unnatural. The "whiteness" it provides is subtle and believable, adding a touch of radiance to my complexion. I get compliments whenever I apply this on my face! The stick format is incredibly easy to apply and reapply throughout the day. It fits perfectly in my purse and doesn't require any messy fingers. The formula absorbs quickly and leaves my skin feeling smooth and protected. I use this sunscreen as a touch-up after applying my regular sunscreen, especially on areas like my face and neck. It provides an extra layer of protection and ensures that I'm fully covered from the sun's harmful rays. If you're looking for a reliable and effective sunscreen that won't leave you feeling greasy or sticky, I highly recommend the Neutrogena Ultra Sheer Dry Touch SPF 50 Mineral Sunscreen Stick. It's a must-have for anyone who wants to protect their skin from the sun while maintaining a healthy, radiant glow.
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Reviewed in the United States on September 8, 2024
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Rita Gonzales
Lexington, US
★★★★★ 5
Comfortable wear
Size: 1.5 Ounce (Pack of 1)
Spreads so easy - the big deodorant dispenser looks weird but it takes fewer swipes. Is soft and you can see where you applied. Must rub it in though. Dries down matte - excellent formula. Spreads easily and sets very quickly. Blurring effect is nice on my old wrinkled face. Not drying or tightening of skin feeling - comfortable and never heavy or tacky unless it’s more than 2 reapplications. Gets too white too. Slight cast but a LOT less than others. It takes more than 2 layers on me (Mac NC 20-25) I think this one is it. Not hard and chalky, agreeable haze but the finish is dry and pores are smoothed over. If they ever made a tinted stick like this I’d feel like I’ve won the lottery
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Reviewed in the United States on May 31, 2025
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KatC
Lowell, US
★★★★★ 4
Does the job, but it does whiten.
Size: 1.5 Ounce (Pack of 1)
It's a nice sunscreen, but I have light skin and it still lightens it even more. It can be blended out to not be as noticeable though. So, if you wanted a hands free sunscreen like I did, this may not work for you since you'd need to blend it out with your hands in order to reduce the whitening effect. Also, you should warm it up with your fingers first before applying it to your face so it doesn't drag your face and glides more easily. Skin wise, no reactions from it. I have slightly sensitive skin and do have acne and it never got worse from using this(used almost daily for 5 months now). If you use makeup, I'm 100% sure this with move your makeup so be careful with that, I'd put it on before and use something else for reapplication after. Texture is smooth and a tad sticky/greasy I'd say. Not 100% matt, but not glossy.
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Reviewed in the United States on June 14, 2024
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Matt
Houston, US
★★★★★ 5
Good sports sunscreen for face! Blends noticeably less than chemical sunscreens
Size: 1.5 Ounce (Pack of 1)
My use case: A sand volleyball tournament that goes from 9am in the morning till 6pm at night in the summer Texas sun. My primary concerns: prevents sunburn, doesn't get in my eyes when sweating. I'm not overly concerned with how well this sunscreen blends in - I don't want an opaque white paste on my face, but I'm also a sweaty sandy mess, so appearance isn't my top priority. For reference, I'm white but tan easily. This sunscreen stick works well for this purpose! I can sweat profusely as you would expect, but there is no stinging in my eyes like I get with chemical sunscreens. It also has broad spectrum protection against UVA and UVB including long wave UVA. If you want a daily sunscreen to prevent aging that appears invisible, this may not be for you. But if you want a good sports sunscreen for face that won't irritate eyes, this is it!
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Reviewed in the United States on June 1, 2026
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Verified Purchase
Anon
San Leandro, US
★★★★★ 5
great sunscreen for face
Size: 1.5 Ounce (Pack of 1)
this is my favorite stick sunscreen for the face. it does have a slight white cast when you first apply it but it goes away once it’s been rubbed into the skin. so many sunscreens say they have a “non-sting formula” but this is the only one i have used that truly doesn’t burn/sting the eyes when applied on the eyelids or when you sweat into your eyes. doesn’t have that traditional “sunscreen smell” either. it leaves a healthy glow to the skin as well. three swipes on all areas of the face is enough coverage. it is a little pricey but i think it lasts quite awhile. highly recommend trying this sunscreen.
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Reviewed in the United States on April 10, 2025

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